In article <854622079.25760 at dejanews.com>, parkstone at lds.co.uk writes
>Hi, I'm an A-level student currently studying chemistry. I am presently
>involved in an extended investigation, which makes up part of my final
snip
>>To measure the rate of reaction, substrate concentration of each
>monosaccharide will be varied and a measurement of carbon dioxide, the
>by-product of fermentation, will be obtained.
>>>If you have any information that you feel could be of use, constructive
>criticism of the proposal or titles and authors of any useful reference
>materials, please e-mail me at richards_ at msn.com>>Thankyou for all your help; I look forward to hearing from you.
>>Felicity Richards
>-------------------==== Posted via Deja News ====-----------------------
>http://www.dejanews.com/ Search, Read, Post to Usenet
Forget about rate-deterimining steps and flux control coefficients
(they're not in your syllabus anyway?). Try this investiagtion, works a
treat:
Using the same experimental approach you've outlined, instead of varying
the sugar type (which you can but interpret it instead in terms of
different transport mechanism across the cell's membrane, it doesn't
matter if its wrong, its a reasonable hypothesis which is what counts in
your grading) vary the concentration of heavy metals, eg try copper,
Lead (works well), and so on. You'll see a nice inhibition of
fermentation and you can us as your hypothesis the possiblity that the
metals are binding to one or more enzymes in the cells and thereby
affecting their rates and hence fermentation.
Herbert
--
Herbert M Sauro
email: HSauro at fssc.demon.co.uk
Telephone: 01974 282428
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