Hello,
I'm looking for experimental examples of protein burden
caused by enzyme overexpression and leading to negative
flux.
Snoep et al. (1995, Microbiology 141:2329-2337) showed
data on negative glycolytic flux in Zymomonas.
Also Quick et al. (1992, Planta 188:522-531) found some
negative flux control coefficients for Rubisco in tobacco
with altered nitrogen supply.
It will be very helpfull for me if someone heared about
other examples of protein burden...
Thanks
Bruno Bost