In a previous message I was writing about control analysis and
channelling. I mentioned one way in which MCA can be applied to
channelling without any problems in terms of the summation theorem.
This is the method developed by Boris Kholodenko and Hans Westerhoff
that uses the elementary reactions of the enzymatic mechanisms.
In that posting I also referred that previously this had been applied
by Cornish-Bowden and collaborator and by myself and collaborators.
Of course I was referring to the use of the elementary reactions, not
its application for the summation theorem or other control analytical
purposes (in fact it was all about the size of metabolite pools).
The papers by Boris and Hans were the first to describe a systematic
way of applying control analyis to channelling mechanisms using the
elementary reactions.
I am sorry for any misunderstanding that the text of my previous
message to this thread may have caused.
As far as I am aware, the other way to apply MCA to channelling (i.e.
seeing the two reactions as a single one) that I suggested has never
been applied. I would be grateful if anyone knows anything to the
contrary (I reply to Danny Kohl's points about this in a separate
message)
--
Pedro Mendes
home: pedro at enzyme.demon.co.uk , work: prm at aber.ac.uk | Aberystwyth,
http://www.enzyme.demon.co.uk/pedro.html | Wales, U.K.
Author of GEPASI, a biochemical kinetics simulator | European
Union
