Paul M. Schlosser wrote:
[...]
> In fact, many of the cellular enzymes we treat as "in solution" are
> contained in membranes, but we can readily treat them with the same
> math.
Not always... See
Nelsestuen, G.L., & Martinez, M.B. (1997)
"Steady State Enzyme Velocities That Are Independent
of [Enzyme]: An Important Behavior in Many Membrane
and Particle-Bound States"
Biochemistry 36 (30) 9081-9086.
However, I would like to know what people think about the nonlinear
Eadie-Hofstee plot in Figure 1B, on which a lot of the discussion seems
to depend... Haven't we all seen similar 'deviant' plots for many
different reasons, including the presence of impurities in substrate(s)?
Petr
_____________________________________________________________
Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA
pkuzmic at biokin.com * http://www.biokin.com * 608.256.1269 fax
