claude aflalo wrote:
> May I take the liberty of mentioning the following publications of
> mine which may be relevant to the issue of the analysis of function for
> localized enzymes:
>> C. AFLALO & N. SHAVIT, 1982. Source of Rapidly Labeled ATP
[snip]
> C. AFLALO, 1997. Localized firefly luciferase probes ATP
Michael Kohn's original question was "How are reaction kinetics affected
by binding to a catalyst (...) to a surface?". I wonder if there is any
possibility that you could kindly distill a concise answer (an
"abstract") from your numerous publications on luciferase. If so, I
myself would be very greatful.
The particular question I am interested in has to do with enzyme
-inhibition-, e.g., by pharmaceutical agents. How relevant are studies
of in-vitro inhibition activity (measurements of inhibition constants in
the solution phase) when the targed enzyme in immobilized? Any example
data?
--Petr
_____________________________________________________________
Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA
pkuzmic at biokin.com * http://www.biokin.com * 608.256.1269 fax
