On Thu, 30 Apr 1998, Petr Kuzmic wrote:
> Michael Kohn wrote:
> > Thank you, Athel and Petr, for your heroic efforts at correcting Pentcho
> > Valev's perennial misinformation. Although I too have been ignoring these
> > missives, influenced by the warning "to remain silent is to lie,"
>> > [skip Mr. Valev's manipulative rubbish evaluation... ]
>> > ...discussing real issues. Some such questions I'd like to
> > see addressed are:
> >
> > a. How are reaction kinetics affected by binding to a catalyst confined to a
> > surface (e.g., enzymes of the endoplasmic reticulum) instead of in
> > solution?
>> That is indeed a very interesting question.
>> [snip...]
>> Are there any good data out there which I could massage with my program
> DYNAFIT (Anal. Biochem. 237, 260, 1996)? I kept looking for some nice
> data in the literature, but not too diligently I must admit. Recently I
> reviewed a paper on channeling but obviously I can't use those data
> (unfortunately the manuscript was rejected).
>> --Petr
>This task is rather difficult since this kind of data is rather
disseminated in time, journals and fields of interest whose overlapping
area (or should I rather say n-dimensional "volume") is not evident.
May I take the liberty of mentioning the following publications of
mine which may be relevant to the issue of the analysis of function for
localized enzymes:
C. AFLALO & N. SHAVIT, 1982. Source of Rapidly Labeled ATP
Tightly Bound to Non-Catalytic Sites on the Chloroplast ATP Synthetase.
Eur. J. Biochem. 126, 61-68.
C. AFLALO & N. SHAVIT, 1983. Steady State Kinetics of Photophos-
phorylation: Limited Access of Nucleotides to the Active Site on
the ATP Synthetase. FEBS Lett. 154, 175-179.
C. AFLALO & N. SHAVIT, 1984. A New Approach to the Mechanism of
Phosphorylation: Modulation of ATP Synthetase Activity by Limited
Diffusibility of Nucleotides Near the Enzyme. Current Topics in Cellular
Regulation 24, 435-445.
C. AFLALO & M. DELUCA, 1987. Continuous Monitoring of
Adenosine-5'-triphosphate in the Microenvironment if Immobilized Enzymes
by Firefly Luciferase. Biochemistry 26, 3913-3920.
C. AFLALO, 1990. Targeting of Cloned Firefly Luciferase to Yeast
Mitochondria. Biochemistry, 29, 4758-4766.
C. AFLALO, 1991. [Invited Review]. Biologically Localized
Firefly Luciferase: A Tool to Study Cellular Processes. International
Reviews in Cytology, 130, 269-323.
C. AFLALO & L.A. SEGEL, 1992. Local Probes and Heterogeneous
Catalysis: A Case Study of A Mitochondria-Luciferase-Hexokinase Coupled
System. J. Theor. Biol. 158, 67-108.
C. AFLALO, 1997. Localized firefly luciferase probes ATP
at the surface of mitochondria. J. Bioenerg. Biomembr. 29, 549-559.
This is by no means a self-promotion spam. I just thought it may help
focussing on Michael Kohn's question [a] above
Claude Aflalo ######################## Phones: Office 972-7-6472118 ####
Dept. of Life Sciences Lab 972-7-6472119
Ben Gurion University of the Negev Fax 972-7-6472890
P.O.Box 653 email: aflaloc at bgumail.bgu.ac.il
Beer Sheva 84105 Israel URL: http://www.bgu.ac.il/life/aflalo.html
