Athel Cornish-Bowden wrote:
>> Petr Kuzmic wrote:
>> >Jannie Hofmeyr wrote:
> >
> >> So, in the sense of calculating control coefficients it is not
> >> important to know which mechanism
...
> >This brings to mind what Box et al. said...
> >
> >Can this be a problem in trying to understand the behavior of
> >biochemical systems over a sufficiently wide range of conditions?...
>> This is pertinent, but it's not the whole story.
...
Agreed 100%. Of course, in asking my question it was not my intention
to debunk the little I know about Metabolic Control Analysis. [God
knows we have "one too many" other people periodically appearing in this
space with that kind of agenda.] In fact, I would point out that George
Box in the textbook I quoted lists both the advantages and
-disadvantages- of mechanistic models (complexity prominently appears in
the latter category).
I was just curious how easy or difficult it is to determine empirical
control coefficients -experimentally- over a sufficient range of
conditions, so as to avoid extrapolation. I -assume- that in modelling
the behavior of a complex biochemical system by using -empirical-
control coefficients, it is considered a standard practice -not- to step
out of concentration ranges that were used to determine those
coeffcients. Am I correct?
-- Petr
_____________________________________________________________
Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA
pkuzmic at biokin.com * http://www.biokin.com * 608.256.1269 fax
