Hi Mark
My question is in reference to a number of kinetic studies
e.g. hydrolysis of p-nirophenylphosphate
(also check out a section in Fersht, Structure and Mechanism)
monitoring of the pre-steady state in the above hydolysis reaction
indicated that there was a rapid pre steady state (burst phase)
and then a slow linear appearance of P when the steady state is attained.
The slow step is attributed to the hydrolysis of the acyl enzyme
intermediate
Therefore, i was wondering if the information obtained from such studies
is similar to that derived from monitoring single turnover reactions
in which [E] >>[S]
most of the data will be concerned with binding rates??
the software development sounds cool
is this concerned with analysis?
i am currently using origin for this
but would not mind experimenting with other packages
l8r, jez
Mark Poolman wrote:
> Jeremy Murray wrote:
>> > I have a question concerning pre steady state kinetics wrt enzyme
> > mechanism.
> > What information can be obtained from such an analysis?
>> If by pre steady state you mean analysis of time course data to extract
> kinetic parameters, and/or compare canditate reaction mechanisms, I am
> working on software that may be of some use. I would be interested in
> hearing a bit more about it.
>> Mark
>> >
