On 14 Dec 99, at 8:35, Bruno Bost wrote:
> I'm looking for informations about studies on in vitro reconstructed
> metabolic pathways, used as tools to perform "in vitro" simulations
> on metabolic control, instead of "in silico" simulations. Does
> anyone hear about such studies ? I would be grateful of any
> information.
You may want to look at:
@ARTICLE{Giersch95,
author = {Christoph Giersch},
year = 1995,
title = {Determining elasticities from multiple measurements of
flux rates
and metabolite concentrations. Application of the multiple
modulation method to a reconstituted pathway},
journal = {Eur. J. Biochem.},
volume = 227,
pages = {194--201}
}
Furthermore, in my own work I performed in vitro experiments on
the bacterial PEP:sugar phosphotransferase system to analyse
its special control properties (it is a group transfer pathway). See:
@ARTICLE{Rohwer_etal98b,
author = {Johann M. Rohwer and Pieter W. Postma and Boris
N. Kholodenko and
Hans V. Westerhoff},
year = 1998,
title = {Implications of macromolecular crowding for signal
transduction and
metabolite channeling},
journal = {Proc. Natl. Acad. Sci. USA},
volume = 95,
pages = {10547--10552}
}
Best regards
Johann Rohwer
--
Johann M. Rohwer
Dept. of Biochemistry, Univ. of Stellenbosch
Private Bag X1, 7602 Matieland
SOUTH AFRICA
Tel.: +27-21-8083029 Fax: +27-21-8083022
E-mail: jr at land.sun.ac.za
WWW: http://www.sun.ac.za/biochem/biochem.html
--
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