Bruno Bost wrote:
> Hello,
>> I'm looking for informations about studies on in vitro reconstructed
> metabolic pathways, used as tools to perform "in vitro" simulations
> on metabolic control, instead of "in silico" simulations.
> Does anyone hear about such studies ?
> I would be grateful of any information.
>> Bruno Bost
>> --
> Bruno Bost, Ph.D.
> INRA Station de genetique vegetale - F-91190 Gif sur Yvette, France
> phone/fax: +33 (0)1 69 33 23 61/40
In our research group we have made a lot of experiments in vitro. See
the above references.
Enrique Melendez-Hevia
La Laguna, Tenerife, Spain
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1. Melendez-Hevia, E., Siverio, J. M. & Perez, J. A. (1984) Studies on
glycolysis in vitro: Role of glucose phosphorylation and
phosphofructokinase activity on total velocity. International Journal of
Biochemistry, 16, 469-476.
2. Siverio, J. M., Torres, N. V. & Melendez-Hevia, E. (1985) Activities
of L-lactate and glycerol phosphate production rates in vitro from
glucose 6-phosphate in regenerating rat liver. International Journal of
Biochemistry, 17, 1015-1017.
3. Torres, N. V., Mateo, F., Melendez-Hevia, E. & Kacser, H. (1986)
Kinetics of metabolic pathways. A system in vitro to study the control
of flux. Biochemical Journal, 234, 169-174.
4. Riol-Cimas, J. M. & Melendez-Hevia, E. (1986) Distribution of
metabolic fluxes towards glycerol phosphate and L-lactate from fructose
1,6-biphosphate in vitro: effect of glycerol phosphate dehydrogenase.
International Journal of Biochemistry, 18, 853-856.
5. Riol-Cimas, J. M. & Melendez-Hevia, E. (1988) Kinetics of metabolic
pathways. Transient response of the glycolytic system after
phosphofructokinase reaction to ADP input. International Journal of
Biochemistry, 20, 29-33.
6. Torres, N. V., Mateo, F., Sicilia, J. & Melendez-Hevia, E. (1988)
Distribution of the flux control in convergent metabolic pathways:
Theory and application to experimental and simulated systems.
International Journal of Biochemistry, 20, 161-165.
7. Torres, N. V., Mateo, F. & Melendez-Hevia, E. (1988) Shift in rat
liver glycolysis control from fed to starved conditions: flux control
coefficients of glucokinase and phosphofructokinase. FEBS Letters, 233,
83-86.
8. Torres, N. V., Souto, R. & Melendez-Hevia, E. (1989) Study of the
flux and transition time control coefficient profiles in a metabolic
system in vitro, and the effect of an external stimulator. Biochemical
Journal, 260, 763-769.
9. Torres, N. V., Mateo, F., Riol-Cimas, J. M. & Melendez-Hevia, E.
(1990) Control of glycolysis in rat liver by glucokinase and
phosphofructokinase: influence of glucose concentration. Molecular and
Cellular Biochemistry, 93, 21-26.
10. Torres, N. V. & Melendez-Hevia, E. (1992) Transition time control
analysis of a glycolytic system under different glucose concentrations.
Control of transition time versus control of flux.
Molecular and Cellular Biochemistry, 112, 109-115.
11. Melendez-Hevia, E., Mateo, F. & Torres, N. V. (1992) Control
analysis of rat liver glycolysis under different glucose concentrations.
Thesubstrate approach and the role of glucokinase. Molecular and
Cellular Biochemistry, 115, 1-9.
12. Lupiañez, J. A., García-Salguero, L., Torres, N. V., Peragón, J. &
Melendez-Hevia, E. (1996) Metabolic support of the flight promptness of
birds. Comparative Biochemistry and Phsiology, 113B, 439-443.
13. Cabezas, H., Raposo, R. R. & Melendez-Hevia, E. (1999) Activity and
metabolic roles of the pentose phosphate cycle in several rat tirssues.
Molecular and Cellular Biochemistry. just published..
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