If you're interested in in vitro reconstitution (a much under used technique
I think) I'd have a look at a series of papers written by an East German
group (as it was then) back in the early 80s, one of the authors was
Schellenberger (I don't have the ref here so I'm afraid I can't be more
specific) but essentially then reconsituted a significant section of
glycolysis and managed to reproduce oscillations and multistationarity - I
thought it was very impressive. The system involved a flow through chamber,
pumping in new enzymes all the time and monitoring the outflow. They were
apparently able to maintain steady state quite easily, they didn't do MCA
but it would have been possible.
Herbert Sauro
Bruno Bost <bost at moulon.inra.fr> wrote in message
news:3.0.1.32.19991214093319.00694944 at moulon.inra.fr...
> Hello,
>> I'm looking for informations about studies on in vitro reconstructed
> metabolic pathways, used as tools to perform "in vitro" simulations
> on metabolic control, instead of "in silico" simulations.
> Does anyone hear about such studies ?
> I would be grateful of any information.
>> Bruno Bost
>> --
> Bruno Bost, Ph.D.
> INRA Station de genetique vegetale - F-91190 Gif sur Yvette, France
> phone/fax: +33 (0)1 69 33 23 61/40
> ---
