What perfect timing, I was looking for material which might explain to me
the ins and outs of modelling enzymes bound to membranes. I shall check out
the refs you mentioned.
Herbert Sauro
"claude aflalo" <aflaloc at bgumail.bgu.ac.il> wrote in message
news:Pine.OSF.4.21.0008220935070.27600-100000 at sansana.bgu.ac.il...
>>> Petr, the alternative, more rigorous approach to membrane-bound enzyme
> kinetics originate basically from the Chemical Engineering field; it
> differentiates between intrinsic (microscopic, as in solution) and
> inherent (solely resulting from their physical state) properties of
> immobilized enzymes. The combination of both yields the effective
> properties of the catalytic system (macroscopic, as observed out of the
> black box).
>> The latter, most often dealt-with by most biologists, yield a distorted
> picture of the system's behavior, especially when the interpretation of
> the results is based on a (tacite) assumption that "ideal" conditions
> prevail...
>> If you're interested please refer to this central (relatively recent)
> review:
> *Engasser, J.-M. and C. Horvath (1976). Appl. Biochem. Bioeng. 1:127-220.
>> A fascinating discussion, pertaining to the effective consequences of
> enzyme organization can also be found at:
> *Ricard, J., N. Kellershohn, et al. (1989). Spatial order as a source of
> kinetic cooperativity in organized bound enzyme systems. Biophys J
> 56:477-487.
>>> Claude Aflalo ########################## Phones: Office 972-7-6472118 ####
> Unit for Science Teaching/Dept. of Life Sciences Lab 972-7-6472119
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