Mosaic analysis with ncl-1

[Jeffrey Yuan]

I am interested in setting up a mosaic analysis experiment using ncl-1 mutant 
worms.  The question I have with the analysis is has anyone had any experience 
using the cosmid (C33C3 if I remember correctly) that rescues ncl-1 instead of 
the duplication qDp3.  Does such an approach work?  I hope to coinject that 
cosmid with a plasmid that rescues my gene, therefore having to avoid linking 
the free duplication to the injected array.

Jeffrey Yuan

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*  Jeffrey Yuan                                                          *
*  Department of Molecular Biology                                       *
*  Lewis Thomas Laboratory                                               *
*  Princeton University                FAX: 609-258-3980                 *
*  Washington Road                   Phone: 609-258-5939                 *
*  Princeton, NJ  08544              email: yuan at phoenix.princeton.edu   *
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