microinjection of DNA
gruidl at MEDSCI.MBP.MISSOURI.EDU
Thu Aug 18 13:37:32 EST 1994
Dear worm netters:
I am getting ready to do microinjection rescue and I want to know if I
need to use CsCl purified DNA. I know about problems with DNA ppt with Na
salts and that I should use K salts. I would guess that there would be
fewer problems with CsCl DNA, but does an alklaine lysis miniprep DNA work OK?
Just a note. As long as I use enough DNA, I am able to do most
manipulations with miniprep DNA including sequencing. My only concern is
how to determine how much DNA I am using or is that not a problem as long
as I have enough and I don't clog the needle.
please email any advise to gruidl at medsci.mbp.missouri.edu
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