Low stringency Hyb conditio
SternM_Lab at QUICKMAIL.CIS.YALE.EDU
Wed Nov 23 11:58:54 EST 1994
Subject: Time:11:38 AM
OFFICE MEMO Low stringency Hyb conditions Date:11/23/94
Hi fellow netters,
I want to probe genomic libraries from other Nematode species with my
genomic fragment from C.elegans.
I have a lot of conflicting information as to how to go about doing this.
I would specifically like to see protocols that have been successful for you
or your lab. I would also like to know what you probed with (genomic, cDNA,
oligo, etc.) and how you might have altered the protocol based on expected
I will happily post a summary of responses if people are interested.
Thanks for your time,
More information about the Celegans