Getting rid of gut autofluorescence

Leon Avery leon at EATWORMS.SWMED.EDU
Tue Oct 25 17:58:04 EST 1994


Does anyone know of a way of getting rid of gut autofluorescence?
Mike Roth and I are trying to stain worms with a fluorescent ceramide
dye.  In cultured mammalian cells this dye gets trapped in the golgi,
so you can use it to look at membrane trafficking and other neat
stuff.  Mike would like to be able to do similar things with C
elegans.  So we fed worms the dye: they eat it, and we think it goes
into intestinal cells.  Unfortunately, gut autofluorescence in both
fluorescein and rhodamine channels makes it impossible to be sure, or
to see where it goes.

Would Flu mutants be any use?  Do the fluorescent granules go away
when you starve the worms?  What the Hell are these granules, anyway?
Lysosomes, maybe?  Some kind of storage granules?

--
Leon Avery					   (214) 648-2420 (office)
Department of Biochemistry			            -2768 (lab)
University of Texas Southwestern Medical Center             -8856 (fax)
5323 Harry Hines Blvd				   leon at eatworms.swmed.edu
Dallas, TX  75235-9038



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