Letter from Morris Maduro, Injection problems: Reply

BAUMEISTER at FRODO.MGH.HARVARD.EDU BAUMEISTER at FRODO.MGH.HARVARD.EDU
Fri Oct 28 07:13:18 EST 1994


Dear Morris,
1. I do not believe that it is lacZ that causes problems.
2. I have tried several preparation methods for DNA to be injected. 
Minipreps which include and RNase treatment have never worked for me,
omitting the RNase helps. Significantly better is a Qiagen prep (obviously,
you get rid of the RNase on the column) or a CsCl prep. Similar results
were obtained by other people in our lab.
These observations are based on 70+ injections.

Good luck

Ralf Baumeister
Ruvkun lab
Mass. General Hospital 



More information about the Celegans mailing list