Letter from Morris Maduro, Injection problems: Reply 2

HART at FRODO.MGH.HARVARD.EDU HART at FRODO.MGH.HARVARD.EDU
Fri Oct 28 08:42:40 EST 1994


Qiagen or CsCl preps are best I agree, but most of us sucessfully inject
DNA preps which are treated with RNase for 1 hour, extracted 1x with
phenol/chloroform, twice with chloroform and then ethanol precipitated. The
yield of F1 progeny and subsequent lines is usually indistinguishable
from Qiagen prep DNA, and all of our cosmid injections are processed this way.

Anne Hart
Kaplan Lab
Mass. General Hospital



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