Using dead E. coli as worm food

Peg MacMorris macmorri at bio.indiana.edu
Tue Sep 27 10:04:51 EST 1994


In article <01HHKHYH2JTUAXB30M at vms.cis.pitt.edu>, LJAC at VMS.CIS.PITT.EDU
wrote:
> 
> Re heat-killed E. coli as worm food:
> 
> About 15 years ago, Dick Russell and I did some careful expts. on this.
> Autoclaving doesn't work well, as the cells clump and the clumps are
> too big for worms to ingest. Milder heat-killing avoids this, but it
> seems that it produces something (only partially removable by washing)
> that inhibits worm growth.
> 
> The best alternative we found was to use streptomycin-killed OP50. At
> concentrations of 200-400 microgm/ml, OP50 is efficiently killed in a
> few hours. The metabolic state of these cells is unclear. The incidence
> of strep-resistant mutant OP50 is low enough not to worry. UV-killing
> is a decent alternative, but a pain for large quantities.
> 
> If someone has a particular objective in mind for using dead E. coli, let
> me know and I might be able to help.
> 
> Lew Jacobson
> Biological Sciences
> Univ. Pittsburgh

Did you ever try microwaving E. coli?  I tried but didn't get adequate
killing of the E. coli and eventually resorted to streptomycin and no
peptone in the agar plates to prevent E.coli growth. 
--
Peg MacMorris         Voice: (812) 855-0102                     
Dept. of Biology                Fax: (812) 855-6705                     
Jordan Hall                Internet: macmorri at bio.indiana.edu 
Indiana University                                                      
Bloomingtion, IN 47405            



More information about the Celegans mailing list