Using dead E. coli as worm food
macmorri at bio.indiana.edu
Tue Sep 27 10:04:51 EST 1994
In article <01HHKHYH2JTUAXB30M at vms.cis.pitt.edu>, LJAC at VMS.CIS.PITT.EDU
> Re heat-killed E. coli as worm food:
> About 15 years ago, Dick Russell and I did some careful expts. on this.
> Autoclaving doesn't work well, as the cells clump and the clumps are
> too big for worms to ingest. Milder heat-killing avoids this, but it
> seems that it produces something (only partially removable by washing)
> that inhibits worm growth.
> The best alternative we found was to use streptomycin-killed OP50. At
> concentrations of 200-400 microgm/ml, OP50 is efficiently killed in a
> few hours. The metabolic state of these cells is unclear. The incidence
> of strep-resistant mutant OP50 is low enough not to worry. UV-killing
> is a decent alternative, but a pain for large quantities.
> If someone has a particular objective in mind for using dead E. coli, let
> me know and I might be able to help.
> Lew Jacobson
> Biological Sciences
> Univ. Pittsburgh
Did you ever try microwaving E. coli? I tried but didn't get adequate
killing of the E. coli and eventually resorted to streptomycin and no
peptone in the agar plates to prevent E.coli growth.
Peg MacMorris Voice: (812) 855-0102
Dept. of Biology Fax: (812) 855-6705
Jordan Hall Internet: macmorri at bio.indiana.edu
Bloomingtion, IN 47405
More information about the Celegans