background problems with Abs

Mike Finney mikef at MJR.COM
Fri Apr 21 18:32:10 EST 1995


>Has preincubating antibodies with fixed worms removed
>contaminating Abs?

Yes.  It even works if you don't have nulls, but if you have the nulls, use
them.  And while you're at it, it never hurts to pre-absorb the
secondaries, either.

You can also affinity-purify the antibodies using a different fusion than
the one you injected, if you didn't do that already.

Mike






More information about the Celegans mailing list