background problems with Abs

[Mike Finney]

>Has preincubating antibodies with fixed worms removed
>contaminating Abs?

Yes.  It even works if you don't have nulls, but if you have the nulls, use
them.  And while you're at it, it never hurts to pre-absorb the
secondaries, either.

You can also affinity-purify the antibodies using a different fusion than
the one you injected, if you didn't do that already.

Mike