Taking pictures of GFP

[Scott Kuersten]

In article <3v9lli$n77 at news.ycc.yale.edu>, RBURDINE at biomed.med.yale.edu
(R.O.S.B.F.) wrote:

> Hi Netters,
> 
> 
> 
> I really have two questions.
> 

> First off,  I ahve GFP staining from a constrcut I injected into e1282 using
> MH86 as my injection marker.  The staining is intense and I need to get some
> pictures of it obviously.  My problem is the staining is COMPLETELY quenched 
> by NaAzide.  


Try anesthetizing the worms with 1mM levamisole.  This was suggested in a
WBG article, probably from the Chalfie or Fire labs.