Taking pictures of GFP
skuerst at bio.indiana.edu
Mon Jul 31 13:14:19 EST 1995
In article <3v9lli$n77 at news.ycc.yale.edu>, RBURDINE at biomed.med.yale.edu
> Hi Netters,
> I really have two questions.
> First off, I ahve GFP staining from a constrcut I injected into e1282 using
> MH86 as my injection marker. The staining is intense and I need to get some
> pictures of it obviously. My problem is the staining is COMPLETELY quenched
> by NaAzide.
Try anesthetizing the worms with 1mM levamisole. This was suggested in a
WBG article, probably from the Chalfie or Fire labs.
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