codon frequency of C. elegans vs. E. coli

Paul N Hengen pnh at fcs260c2.ncifcrf.gov
Thu Jun 1 12:20:42 EST 1995


What is the difference between the codon frequencies of C. elegans vs. E.
coli?  Would one expect a cDNA cloned fragment from C. elegans, which has been
6xHis- tagged and expressed in E. coli, to possess rare codons which might
cause stalling of the ribosome and premature termination of translation
leading to smaller peptides than that of the fusion product?

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* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
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