strange behaviour of integrant

Morris Maduro Morris_maduro at biology.ualberta.ca
Thu Mar 9 06:41:44 EST 1995


Hello once again,

Thanks to all those who sent replies to my inquiries regarding integration
of transgenic arrays.  I screened 300 plates of cloned F2's and recovered a
strain that gives 100% transmission.  The original array consisted of pRF4
and an unc-119::GFP fusion plasmid.  All of the animals of this strain
fluoresce and all are right-handed rollers.

I crossed a few of these putative integrants with N2 males, and almost all
the progeny failed to roll.  Those that did (only a few) rolled very
weakly.  All of the F1's did fluoresce, however, although apparently
slightly weaker than the parent strain.  In the F2, the strong right-hand
rol phenotype resegregated, and of those, all fluoresced, and brighter than
the F1's.

It looks like the rol-6D (su1006) has become recessive, maybe due to some
kind of position effect.  Either that, or I've integrated the array
_without pRF4_ into a rol gene.  (Of course, the apparent recessive nature
of the rol-6 marker makes crossing the 'integrant' easier since the F1
males mate well.  Also it will be easier to map it.)

Has anyone else had this happen?  As far as I know the F1s should all roll
if it is a true integrant.  I'm in the process of backcrossing the F1 males
to the original 'integrant' strain to test for recessive rol.

I'd appreciate any comments.

MM
-- 
Morris Maduro
Dept of Biological Sciences
University of Alberta
Edmonton, AB Canada          Morris_maduro at biology.ualberta.ca



More information about the Celegans mailing list