liquid drops in lids of worm plates -> loss of worms

burglin at ubaclu.unibas.ch burglin at ubaclu.unibas.ch
Thu Mar 9 19:14:06 EST 1995


Hi,
a very simple, but sometimes quite important issue:
Liquid drops in the lid of worm plates.
I would like to know what your experiences are with
storing and incubating worm plates.
We tend to get a lot of liquid in the lids.  Clearly
the overall "wetness" of the plates plays a role.  Nevertheless,
storage conditions (cold room, or r.t.) play a role too.
Clearly plates accumulate more liquid in the lid when they are 
in the top and the bottom of a stack of plates.
The problem with the liquids in the lid is that often many
worms (the dauers) crawl up the side of the plate into the droplets, and
once the droplets dry out, the worms die.  So your popluation on
the plate does not survive that well, since most worms died up in the lid.
Have people found conditions that reduce liquid drops in the lid?
In the 60% humidity worm room in Gary Ruvkun's lab this was virtually no
problem, but in incubators it is a lot more pronounced, perhaps
because of circulating air, and temperature differentials?
(even though the plates are in boxes)
Do you just ignore this, or have you any simple solutions?
Thanks
Thomas Burglin
burglin at ubaclu.unibas.ch
 



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