gfp/fixation

Dave Pilgrm dave_pilgrim at biology.ualberta.ca
Tue Nov 14 01:21:36 EST 1995


Ikue Mori wrote,
> Does anyone know if gfp can still work after the worms were fixed for
> antibody or lacZ staining? I know by reading one of Marty Chalfie's Gazette
> articles that gfp works after formaldehyde and/or glutaraldehyde fixation.
> So, I gather that the worms can be "doubly stained" with gfp and DAPI (or 
> lacZ fixation, perhaps?). My question may be a stupid one since, to my
> knowledge, nobody has tried to observe cells expressing gfp and an antibody
> (or lacZ) in a single animal. However, I would appreciate to hear any
> comments from anyone who had thought about the same attractive possibility.


Seems to work fine. We have seen nice double staining with GFP/DAPI, and
Chris Link has looked at unc-119:GFP / anti-synaptotagmin (I think he
showed some photos at the worm meeting)

-- 
Dave Pilgrim                 | 
Dept of Biological Sciences  |    
University of Alberta        |
Edmonton, AB Canada          | dave_pilgrim at biology.ualberta.ca



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