Blue Fluorescent Protein

Paul Baum baum at
Sat Aug 24 14:27:39 EST 1996

We would like to use blue fluorescent protein for double labeling
alongside GFP.  Has anyone in the worm community tried using the
double-mutant BFP protein Y66H Y145F described by Heim and Tsien in
Current Biology 1996 vol. 6 no. 2?  Can the fluorescence be distinguished
from GFP by using normal or special filter sets?  Is the bleaching so bad
that the BFP signal cannot be detected with high speed film or a CCD



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