GFP

EVAN MULLIGAN mulligan at UMBSKY.CC.UMB.EDU
Wed Feb 7 17:41:52 EST 1996


I recently posted a question about visualizing and counting two different
strains being grown in the same culture: a number of people replied (thanks
to you all) and some suggested that one of the strains could be transformed
with the GFP gene.  My new question is, will a strain transgenic with GFP
continue to express the gene indefinitely, or will it disappear over a
number of generations?  

Also, I was reminded that two strains living in the same culture will cross
breed (due to males); will vigorous shaking of a liquid culture prevent this
from occurring, or will it merely reduce the frequency with which it occurs 
(perhaps to a negligible amount)?

Lastly, if one CAN indefinitely maintain GFP in a strain, where could such
a strain be obtained?  (We have a microinjection facility here at UMass, but
not a very advanced one; plus, I have absolutely no experience in this area,
so I thought I'd try and sponge some GFP worms off someone else, if I may.)

Thanks
Evan Mulligan
UMass/Boston
100 Morrissey Blvd.
Boston MA 02125
(617) 287-6585
mulligan at umbsky.cc.umb.edu



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