wizard columns ok for cosmids?

Chris Thorpe thorpe at molbio.uoregon.edu
Thu Jan 4 18:21:06 EST 1996


 Well, the subject line pretty much says it all.  I and some others in
Bruce Bowerman's lab are attempting to get rescue of a couple mutants by
injection of cosmid DNA.  We use a Wizard column to clean up the DNA
following a standard alkyline lysis prep.  Has anyone out there used one
of these columns for preparing DNA for injection? Are the preps clean
enough for efficient transformation?   Thanks for any help.



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