wizard columns ok for cosmids?
thorpe at molbio.uoregon.edu
Thu Jan 4 18:21:06 EST 1996
Well, the subject line pretty much says it all. I and some others in
Bruce Bowerman's lab are attempting to get rescue of a couple mutants by
injection of cosmid DNA. We use a Wizard column to clean up the DNA
following a standard alkyline lysis prep. Has anyone out there used one
of these columns for preparing DNA for injection? Are the preps clean
enough for efficient transformation? Thanks for any help.
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