help with a biochemistry lab

Bruce Wightman wightman at mendel.berkeley.edu
Mon Jun 10 13:26:47 EST 1996


I was wondering if anyone could provide suggestions for good proteins to
partially purify/purify for an introductory biochemistry lab for
undergraduates.  I'd like to be able to give students an experience with
purification by gel filtration, ion exchange, and/or affinity
chromatography.  Preferably, the protein should have an activity that can
be assayed easily and cheaply.  The starting material should be something
that's clearly recognizable as "life".  Unfortunately, the number of steps
has to be very limited, as time does not allow anything particularly
complicated.  Also, it has to be doable at room temperature.  

Any ideas, good references, or protocols?

Thanks-
Bruce Wightman
Department of Molecular and Cell Biology
401 Barker Hall- Garriga Lab
University of California
Berkeley, CA   94720-3204



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