help with a biochemistry lab

Ron Tate rtate at bmb-fs1.biochem.okstate.edu
Mon Jun 10 16:19:07 EST 1996


Bruce Wightman wrote:
> 
> I was wondering if anyone could provide suggestions for good proteins to
> partially purify/purify for an introductory biochemistry lab for
> undergraduates.  I'd like to be able to give students an experience with
> purification by gel filtration, ion exchange, and/or affinity
> chromatography.  Preferably, the protein should have an activity that can
> be assayed easily and cheaply.  The starting material should be something
> that's clearly recognizable as "life".  Unfortunately, the number of steps
> has to be very limited, as time does not allow anything particularly
> complicated.  Also, it has to be doable at room temperature.
> 
> Any ideas, good references, or protocols?

How about lysozyme from egg white, its pretty easily purified by ion 
exchange and the assay is pretty easy too.

Just another suggestion, its done here in an undergraduate lab course.

-- 
>>>>>>--------------------------->
>Ron Tate                                                                   
>Lab of Franklin Leach
>Dept. of Biochem. & Molecular Biology
>Oklahoma State University rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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