fusion versus peptide antibody

Oliver Hobert hobert at FRODO.MGH.HARVARD.EDU
Wed Nov 13 16:34:40 EST 1996


To every worm-breeder who ever made an antibody:

We have contineous debates in our lab (based more on speculations than on
real facts) about which antibodies work better (or at all) on C.elegans
whole mount stains: antibodies raised against fusionproteins (GST, MBP
etc.) or antibodies raised against synthetic peptides.
Could everybody who ever made an antibody and tried it on worms send me a
note whether a anti-fusion antibody worked or didn't work or an
anti-peptide antibody worked or didn't work. I will assemble all answers
into a "statistic" and send it around.
Thanks all,

Oliver

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Oliver Hobert
Harvard Medical School/Department of Genetics
Mass. General Hospital/Department of Mol. Biol.
Ruvkun-Lab
Wellman 8
Boston, MA 02114
Fax: 1-617-726 6893
Tel: 1-617-726 5983
e-mail: hobert at molbio.mgh.harvard.edu
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