overexpression in C. elegans

Edward Kipreos ekipreos at cb.uga.edu
Sun Nov 17 00:23:01 EST 1996


I would like to have a means for obtaining overexpression from cDNAs. Does
anyone have experience with promoters that could be used for constitutive
or inducible overexpression in all cells. While the levels required for
overexpression are going to be different between genes (as the normal
expression levels vary), I would appreciate hearing of any experiences that
people have had with overexpression from various promoters.

Thanks,

Edward

---------------------------------
Edward T. Kipreos
Department of Cellular Biology
The University of Georgia
353 Biological Sciences Building
Athens, GA  30602-2607
office: (706) 542-3862
lab:    (706) 542-3137
fax:    (706) 542-4271
email:  ekipreos at cb.uga.edu
---------------------------------





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