Egggs RNA extraction

Jean-Claude LABBE labbej at MERCK.BCH.UMONTREAL.CA
Tue Nov 19 10:44:29 EST 1996


Dear worm breeders,

	I recently extracted RNA from a bunch of different strains at 
different stages, using the protocol published in WBG 13(3) by Rebecca 
Burdine and Michael Stern.  While, at the end, I had no problem 
resuspending the RNA from most of the strains, I had a very hard time to 
resuspend RNA extracted from C. elegans N2 eggs, althought they were 
treated the same way as the others preps.  Has anybody ever experienced 
such a phenomenon, or is it only a simple coincidence that only the egg 
prep went weird?

Thanks,

Jean-Claude Labbe
Departement de biochimie
Universite de Montreal



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