T/A PCR cloning

gbga14 at udcf.gla.ac.uk gbga14 at udcf.gla.ac.uk
Fri Nov 29 05:51:23 EST 1996


Does anyone have a method for adding a single T to the OH end of a blunt 
cut plasmid to permit cloning of Tac amplified PCR products directly.  I 
assume it is possible using a dideoxy T and terminal transferase, 
however an actual method that is known to work would be useful.  
-- 

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This message is from:

Iain Johnstone
Wellcome Unit of Molecular Parasitology, University of Glasgow
Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland

Tel: 44 141 339 8855 Ext 2000
Fax: 44 141 330 5422
email: i.johnstone at udcf.gla.ac.uk


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