Anesthetize to see GFP

Johnny Fares fares at cuccfa.ccc.columbia.edu
Fri Apr 25 12:18:17 EST 1997


     I was wondering if people had a different way to anesthetize worms to
view GFP and be able to recover the worms.  As is known, sodium azide
quenches GFP fluorescence.  I have been using 1% formaldehyde which works
well but which doesn't allow for the recovery of the worms.  At this
point, I would like to laser ablate some cells that are marked by GFP, and
recover the worms; ergo the dilemna.  I have heard that ethanol works?
     Please address any answers to my account if possible and I will later
on post a summary of what is available.

Johnny Fares
701 West 168 Street
HHSC Room 718
Dept. of Biochemistry
Columbia University
New York, NY, 10032

Tel: 212 305 6931
Fax: 212 305 1721





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