longevity in C. elegans

Vladimir V. Bakaev vlad at bakaev.nsk.ru
Tue Aug 19 06:39:29 EST 1997


>From The Worm Breeder's Gazette, 1995, v. 14, No. 1, p. 102.

      THE EFFECT OF INTERMITTENT TEMPERATURE ON LONGEVITY
               AND FECUNDITY OF C. ELEGANS
  Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR

It is well known that low temperatures can prolong  longevity
of  different  animals.  In this study the experimental worms
were mantained in liquid medium with E.  coli in +21oC during
the  day (8 - 12 hrs) and in +4oC during the night,  in dark-
ness.  One control group was mantained  in  +21oC  and  other
control group was mantained in +4oC constantly.  The obtained
results   are   presented    in    the    following    table.
─────────────────────────────────────────────────────────────
            Control group    Experimental   Control group
               (+21oC)          group          (+4oC)
              Mean+/-S.D.     Mean+/-S.D.   Mean+/-S.D.
────────────────────────────────────────────────────────────
Mean longe-  19,86+/-1,63    22,96+/-1,57   38,3+/-2,72
vity (days)     (n = 22)        (n = 24)      (n = 22)
─────────────────────────────────────────────────────────────
Maximal lon-
gevity (days)      34               35            50
────────────────────────────────────────────────────────────
Minimal lon-
gevity (days)       6               10             5
─────────────────────────────────────────────────────────────
Mean         76,91+/-4,54    54,33+/-3,32  4,54+/-2,07
fecundity       (n = 22)         (n = 24)      (n = 22)
─────────────────────────────────────────────────────────────
Maximal           118               95            46
fecundity
─────────────────────────────────────────────────────────────
Minimal
fecundity          33               25             0
─────────────────────────────────────────────────────────────
It can be concluded that such intermittent temperature is not
able to prolong the life-span of C. elegans significantly, in
comparison with constant cold,  as well as fecundity.
Acknowledgment:  The author wishes to express his thanks to
CGC for providing C. elegans (wild line) and E. coli OP50.




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