OP50.1

[Carlos Eduardo Winter]

Dear coleagues,

Upon reading your e-mail I remembered that we have made an electrophoresis
of NA22 (which some people also use as C. elegans food) plasmids. It has
a big plasmid of 50 MDa. Did you check for plasmids in OP50?

Good hunting,

Carlos E. Winter

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
  CARLOS EDUARDO WINTER - Department of Parasitology - ICB2 - USP
  Av. Prof. Lineu Prestes, 1374 - 05508-900 Sao Paulo - BRAZIL
  FAX: (55)(11)818-7417 - PHONE: (55)(11)818-7269
  http://www.icb2.usp.br/~cewinter/
----------------------------------------------------------------------- 
  The human mind treats a new idea the way the body treats a strange
  protein -- it rejects it. - Sir Peter B. Medawar
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

On 26 Aug 1997 fodoran at FALCO.GEOBIO.ELTE.HU wrote:

> On Tue, 26 Aug 1997 fodoran at ludens.elte.hu wrote:
> 
> Dear Liz, Dear Newsgroup,
> 
> Although we are going to send these data to the next issue of the
> Newsletter, let us share our results with you:
> 
> 			ANTIBIOTICS SENSITIVITY OF OP50.
> 
> Csaba Bognar(2), Tibor Vellai(1) and Andras Fodor(1),
> 1. 	Dept. Genetics, Eotvos University, H-1088 Budapest, Muzeum krt. 4/A,
> 	Hungary
> 2. 	Bela Johann National Institute of Public Health in Hungary, Department
> 	of Bacteriology, (OKI), H-1097 Budapest, Gyali ut 2-6, Hungary.
> 
> E. coli OP50 is used practically by everybody in the C. elegans Community
> as natural food for C.elegans. Our substrain what we use was kindly
> provided by Ian Hope in 1995. We have tested its antibiotics sensitivity,
> by the paper disc method routinly used the OKI. We used 30 ug/ml of each
> antibiotics tested. The filter paper discs are out on the center of the
> NGM plate seeded by OP bacteria. An inhibition zone develops, if the
> antibiotics is effective against the baccteria under test. Conventionally,
> if the diameter oif the inhibition zone is smaller then 2 cm, we conclude
> that the bacteria is resistant to the antibiotics tested. We tested 24
> antibiotics, as follows:
> 
> gentamycin; tobramycin; sumetrolim; chloramphenicol; tetracycline;
> ampicyllin; karbenicillin; cefalexine; cefamandol; cefuroxime; amykacin;
> netylmycine; azlocilline; mezlocillin; ceftriaxone; cefatoxim;
> cefroperazon; ofloxacin; pestloxacin; cyprofloxacim; ceftazidin; cefaclor;
> augmentin; and kanamycin. (Some of the names are probably written in very
> Hungarian; we promise the correct them in the Newsletter article).
> 
> Well, we found that OP50 was completely resistant to all these
> antibiotics.
> 
> I hope these data help Liz and other fellow Colleauges to work with OP50.
> 
> Faithfully yours,
> 
> Andras and his gang.   
> 
> > From:	MX%"ryder at WPI.EDU" 14-AUG-1997 16:32:41.88
> > To:	MX%"bionet-news at dl.ac.uk"
> > CC:	
> > Subj:	Re: OP50 Antibiotic resistance
> > 
> > Return-Path: <server-daemon at dl.ac.uk>
> > Received: from mserv1.dl.ac.uk by ludens.elte.hu (MX V4.2 VAX) with SMTP; Thu,
> >           14 Aug 1997 16:32:41 +0100
> > Received: by mserv1.dl.ac.uk id PAA03450 (8.6.13/5.3[ref postmaster at dl.ac.uk]
> >           for dl.ac.uk from server-daemon at dl.ac.uk); Thu, 14 Aug 1997 15:18:51
> >           +0100
> > Resent-From: <server-daemon at dl.ac.uk>
> > Received: by mserv1.dl.ac.uk id PAA03350 (8.6.13/5.3[ref postmaster at dl.ac.uk]
> >           for dl.ac.uk from server-daemon at dl.ac.uk); Thu, 14 Aug 1997 15:17:51
> >           +0100
> > Received: by mserv1.dl.ac.uk id PAA03112 (8.6.13/5.3[ref postmaster at dl.ac.uk]
> >           for dl.ac.uk from news at dl.ac.uk); Thu, 14 Aug 1997 15:16:17 +0100
> > Date: 14 Aug 1997 06:56:36 -0700
> > Resent-Date: Thu, 14 Aug 97 15:18:40 UT
> > Message-ID: <97814151840.~INN-LWAa00195.bionet-news at dl.ac.uk>
> > Resent-Message-ID: <Pine.OSF.3.95q.970814095639.29121B-100000 at bert.WPI.EDU>
> > Precedence: list
> > From: ryder at WPI.EDU (Elizabeth Ryder)
> > Reply-To: ryder at WPI.EDU (Elizabeth Ryder)
> > Sender: "bionet.celegans mail newsgroup" <server-daemon at dl.ac.uk>
> > To: "bionet.celegans mail newsgroup" <bionet-news at dl.ac.uk>
> > Subject: Re: OP50 Antibiotic resistance
> > Comments: List problems/queries to <biosci at dl.ac.uk>
> > Comments: To mail both the group and netnews send to (celegans at dl.ac.uk)
> > X-Article-Number: bionet.celegans Msg # 1646
> > X-Listpath: bionet-news
> > X-Mailer: MXT V 12.16.1
> > 
> > All I know is that it's NOT resistant to Amp or Kan.
> > 
> > Liz Ryder
> > Worcester Polytechnic Institute
> > Dept. of Biology and Biotechnology
> > Worcester, MA  01609
> > email:  ryder at wpi.edu
> > phone:  508-831-6011
> > fax:    508-831-5936
> > 
> > On Wed, 13 Aug 1997, John M. Hawdon wrote:
> > 
> > > Hi;
> > > 	Anyone off hand know whether OP50 is resistant to any antibiotics?
> > > 
> > > Thanks
> > > 
> > > John Hawdon
> > > 
> > > 
> > 
> > 
> > 
> > 
> 
> ========================================================================
> Dr. Andras Fodor			e-mail: fodoran at ludens.elte.hu	
> Associate Professor			or:	fodoran at falco.elte.hu
> Eotvos University			Phone:	+36.1.266.1296
> Department of Genetics				+36.1.267.5444 ext. 2348
> Budapest, Muzeum krt. 4/A		FAX:	+36.1.266.2694
> H-1088 HUNGARY
> ========================================================================
> 
> 
>