OP50.1 (fwd)

fodoran at FALCO.GEOBIO.ELTE.HU fodoran at FALCO.GEOBIO.ELTE.HU
Wed Aug 27 01:39:49 EST 1997


Dear Colleauges,

Your friend Andras has probably a starting Altzheimer or a other kind of
brain lesion. I changed the word SENSITIVE to RESISTANT!!!!

Sorry!

So OP50 PROVED EXTREMELY  

				SENSITIVE,

(not resistant)				TO ALL THE 24 antibiotics teste.

Faithfuly yours, 

	Andras (without his gang).
========================================================================
Dr. Andras Fodor			e-mail: fodoran at ludens.elte.hu	
Associate Professor			or:	fodoran at falco.elte.hu
Eotvos University			Phone:	+36.1.266.1296
Department of Genetics				+36.1.267.5444 ext. 2348
Budapest, Muzeum krt. 4/A		FAX:	+36.1.266.2694
H-1088 HUNGARY
========================================================================


---------- Forwarded message ----------
Date: Tue, 26 Aug 1997 20:37:01 +0200 (MET DST)
From: fodoran at falco.geobio.elte.hu
To: "Bionet.Celegans.Mailgroup" <biosci at dl.ac.uk>,
    Netbionet Celegans <celegans at net.bio.net>,
    Theresa Stierngale <stier at molbio.cbs.umn.edu>,
    Tibor Vellai <vellai at falco.geobio.elte.hu>, ryder at WPI.EDU
Cc: FODORAN at FALCO.elte.hu
Subject: Re: OP50.1

On Tue, 26 Aug 1997 fodoran at ludens.elte.hu wrote:

Dear Liz, Dear Newsgroup,

Although we are going to send these data to the next issue of the
Newsletter, let us share our results with you:

			ANTIBIOTICS SENSITIVITY OF OP50.

Csaba Bognar(2), Tibor Vellai(1) and Andras Fodor(1),
1. 	Dept. Genetics, Eotvos University, H-1088 Budapest, Muzeum krt. 4/A,
	Hungary
2. 	Bela Johann National Institute of Public Health in Hungary, Department
	of Bacteriology, (OKI), H-1097 Budapest, Gyali ut 2-6, Hungary.

E. coli OP50 is used practically by everybody in the C. elegans Community
as natural food for C.elegans. Our substrain what we use was kindly
provided by Ian Hope in 1995. We have tested its antibiotics sensitivity,
by the paper disc method routinly used the OKI. We used 30 ug/ml of each
antibiotics tested. The filter paper discs are out on the center of the
NGM plate seeded by OP bacteria. An inhibition zone develops, if the
antibiotics is effective against the baccteria under test. Conventionally,
if the diameter oif the inhibition zone is smaller then 2 cm, we conclude
that the bacteria is resistant to the antibiotics tested. We tested 24
antibiotics, as follows:

gentamycin; tobramycin; sumetrolim; chloramphenicol; tetracycline;
ampicyllin; karbenicillin; cefalexine; cefamandol; cefuroxime; amykacin;
netylmycine; azlocilline; mezlocillin; ceftriaxone; cefatoxim;
cefroperazon; ofloxacin; pestloxacin; cyprofloxacim; ceftazidin; cefaclor;
augmentin; and kanamycin. (Some of the names are probably written in very
Hungarian; we promise the correct them in the Newsletter article).

Well, we found that OP50 was completely SENSITIVE to all these
antibiotics.

I hope these data help Liz and other fellow Colleauges to work with OP50.

Faithfully yours,

Andras and his gang.   

> From:	MX%"ryder at WPI.EDU" 14-AUG-1997 16:32:41.88
> To:	MX%"bionet-news at dl.ac.uk"
> CC:	
> Subj:	Re: OP50 Antibiotic resistance
> 
> Return-Path: <server-daemon at dl.ac.uk>
> Received: from mserv1.dl.ac.uk by ludens.elte.hu (MX V4.2 VAX) with SMTP; Thu,
>           14 Aug 1997 16:32:41 +0100
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>           for dl.ac.uk from news at dl.ac.uk); Thu, 14 Aug 1997 15:16:17 +0100
> Date: 14 Aug 1997 06:56:36 -0700
> Resent-Date: Thu, 14 Aug 97 15:18:40 UT
> Message-ID: <97814151840.~INN-LWAa00195.bionet-news at dl.ac.uk>
> Resent-Message-ID: <Pine.OSF.3.95q.970814095639.29121B-100000 at bert.WPI.EDU>
> Precedence: list
> From: ryder at WPI.EDU (Elizabeth Ryder)
> Reply-To: ryder at WPI.EDU (Elizabeth Ryder)
> Sender: "bionet.celegans mail newsgroup" <server-daemon at dl.ac.uk>
> To: "bionet.celegans mail newsgroup" <bionet-news at dl.ac.uk>
> Subject: Re: OP50 Antibiotic resistance
> Comments: List problems/queries to <biosci at dl.ac.uk>
> Comments: To mail both the group and netnews send to (celegans at dl.ac.uk)
> X-Article-Number: bionet.celegans Msg # 1646
> X-Listpath: bionet-news
> X-Mailer: MXT V 12.16.1
> 
> All I know is that it's NOT resistant to Amp or Kan.
> 
> Liz Ryder
> Worcester Polytechnic Institute
> Dept. of Biology and Biotechnology
> Worcester, MA  01609
> email:  ryder at wpi.edu
> phone:  508-831-6011
> fax:    508-831-5936
> 
> On Wed, 13 Aug 1997, John M. Hawdon wrote:
> 
> > Hi;
> > 	Anyone off hand know whether OP50 is resistant to any antibiotics?
> > 
> > Thanks
> > 
> > John Hawdon
> > 
> > 
> 
> 
> 
> 

========================================================================
Dr. Andras Fodor			e-mail: fodoran at ludens.elte.hu	
Associate Professor			or:	fodoran at falco.elte.hu
Eotvos University			Phone:	+36.1.266.1296
Department of Genetics				+36.1.267.5444 ext. 2348
Budapest, Muzeum krt. 4/A		FAX:	+36.1.266.2694
H-1088 HUNGARY
========================================================================






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