OP50 follow up
Tritech_Research at lamg.com
Wed Aug 27 19:31:58 EST 1997
has anyone ever tried to transform the OP50 strain with any plasmid
containing an antibiotic resistant marker?
Yes - it transforms easily with pUC19 / Bluescipt type plasmids
and is then resistant to Amp as expected. However, I believe it is
"blue" with or without a plasmid, since it is wild-type for B-gal,
and it doesn't transform as efficiently as MC1061, DH5a, etc.
tritech_research at LAMG.COM
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