worm DNA preps

Leon Avery leon at EATWORMS.SWMED.EDU
Fri Jun 20 15:11:10 EST 1997


I am making a frozen library of mutagenized worms for reverse
genetics.  To use this library most efficiently it will be necessary
to do a lot of worm DNA preps (1000, for instance).  If possible I
would like DNA sufficiently pure to be cut efficiently by restriction
enzymes.  Since I don't want to do 1000 phenol extractions, I'm
thinking about using one of the DNA purification kits (Wizard, Qiagen,
Promega...) that can be obtained in a 96-well format.

Does anyone have any experience purifying worm DNA with one of these
systems (not necessarily in 96-well format)?

Thanks,
Leon Avery                                       (214) 648-4931 (voice)
Department of Molecular Biology and Oncology              -1488 (fax)
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd                            leon at eatworms.swmed.edu
Dallas, TX  75235-9148                  http://eatworms.swmed.edu/~leon/



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