Clean antibodies

LJAC at VMS.CIS.PITT.EDU LJAC at VMS.CIS.PITT.EDU
Wed Mar 5 14:22:18 EST 1997


We have a constant problem with secondary (anti-mouse) Ab's that react with a
limited number of worm proteins in the absence of primary Ab. We've frequently
had this problem with rabbit anti-mouse from various suppliers and have now
encountered the problem with goat anti-mouse. I presume this occurs because the
rabbits (or goats) are infected with some kind of nematode parasite.

Does anyone have a favorite supplier that reliably provides anti-mouse Ab with
little anti-worm reactivity? Does anyone have experience (or a suspicion) that
goat or sheep would be better than rabbit in this regard?

Lew Jacobson
Biological Sciences
Univ. of Pittsburgh
LJAC at vms.cis.pitt.edu





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