cell fraction markers
jeb.gaudet at MAILBOX76.UTCC.UTORONTO.CA
Wed May 7 07:12:15 EST 1997
I've recently been performing cell fractionation experiments to look at
the localisation of my favourite protein by Western blot, and I need a way
to verify that the fractionation is working.
Specifically, I'd like to use antibodies against worm proteins that are
known to localize to specific cell compartments, in order to show that my
biochemical fractions correspond to particular cellular fractions.
I need indicators for cytosol, membrane, and cytoskeletal fractions, as
well as for nuclei. Does anyone know of any commercially-available
antibodies that would be of use? Or does anyone have antibodies which
they'd be willing to share?
I can be reached at the e-mail address below. Any help would be greatly
Dept. of Medical and Molecular Genetics
University of Toronto
jeb.gaudet at utoronto.ca
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