mating into vulvaless animals

daemon at net.bio.net daemon at net.bio.net
Tue Oct 28 11:21:48 EST 1997


Hi everyone.
We have a putative knock-out, but the animals look vulvaless
and bag.
We need to backcross this.

What is the current state-of-the art for mating
into vulvaless animals?

I remember people mention poking holes into
the hermaphrodite to allow male mating.
What's the best way to do this?

Also, how about injection sperm directly?
Is this done these days? What protocol to make sperms?


Thanks everyone in advance.
Thomas



* Thomas Burglin (international character set: B=FCrglin)
* Dept. of Cell Biology,
* Biozentrum, University of Basel
* Klingelbergstrasse 70
* CH-4056 Basel
* Switzerland
* +41 61 267-2066 / +41 61 267-2067 / fax: +41 61 267-2078





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