Thomas Burglin's need to back cross a vul mutant

James A. Waddle jwaddle at sequencer.wustl.edu
Wed Oct 29 12:05:16 EST 1997


>Thomas Burglin writes:
>We have a putative knock-out, but the animals look vulvaless
>and bag.
>We need to backcross this.

Thomas, as an alternative to poking holes in the worm and artificial
insemination, you may, if the mutant is not too sickly, try heat shocking
to increase the frequency of spontaneous males and then mate to N2
hermaphrodites or tra-2(q122) females.

-Jim Waddle





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