making males

Elizabeth Ryder ryder at WPI.EDU
Wed Oct 29 21:56:42 EST 1997


Hi--
	We  don't seem to be having great success producing males by heat
shock, and wonder if anyone has come up with improvements on the heat
shocking protocol, or any other ways of producing males.  The protocols we
have tried are:

1) put L4s/young adults at 30 degrees for 6 hours
2) put L4s/young adults at 27.5 degrees for 30 hours

We then move worms back to 20 degrees and look at progeny for males.
Does anyone do anything different?
	Thanks!
	Liz

Liz Ryder
Worcester Polytechnic Institute
Dept. of Biology and Biotechnology
Worcester, MA  01609
email:  ryder at wpi.edu
phone:  508-831-6011
fax:    508-831-5936





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