transcription start point

wilson fb6x001 at
Tue Sep 9 14:02:55 EST 1997

Thank you very much Jim, but I wonder how this is possible. Did you
detect trans-spliced and non-spliced gene products? I gather some genes
may, or may not be trans-spliced, but i think just a small proportion of
genes in C. elegans behave this way. Maybe it could just have been the
case with your rol-6 gene? What if all mRNAs are trans-spliced? Any
ideas? Maybe working from the genomic DNA?

Jim Kramer wrote:
> We determined the transcription start point for the tans-spliced rol-6
> cuticle collagen gene by primer extension with a primer upstream of the
> splice acceptor site.
> wilson wrote:
> > Hello netters,
> > I got a burning question here for suggestions. Has any work been done on
> > the determination of transcription start point of trans-spliced gene in
> > C. elegans or any other organism whatsoever? If not, what available
> > methods can possibly be used to overcome this?
> > Thanx!!!
> >
> > Wilson

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