lysing worms for biochemistry

patterson at FRODO.MGH.HARVARD.EDU patterson at FRODO.MGH.HARVARD.EDU
Tue Sep 16 11:00:25 EST 1997


I am interested in getting worm lysate for co-immunoprecipitation and
the like, and I am wondering if anyone has suggestions on preparing the
worms.  Is freeze-thawing adequate, or should we add sonication or other
mechanical disruption?

Garth Patterson
Ruvkun Lab



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