longevity in C. elegans 6

daemon at net.bio.net daemon at net.bio.net
Fri Sep 26 11:06:39 EST 1997


>From The Worm Breeder's Gazette, 1996, v. 14, No. 2, p. 33.

AN ATTEMPT TO SLOW AGING IN C. elegans. 3.THE EFFECT OF
EXTRACT FROM YOUNG WORMS DURING POSTREPRODUCTIVE PERIOD
 Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR

C. elegans (1 - 2 days old)  were  collected  from  separate
stock,  frozen  and  after  12  hours or more time,  thawed,
filtrated and mixed with S medium containing E.  coli (1:1),
this  mixture was used as a medium for worms in experimental
group. The medium for control group was prepared by mixing S
medium  containing  E.  coli  with S medium without E.  coli
(1:1).  Three adult animals (3 - 5 days old)  were  kept  in
microtitre  wells  containing 0,75 ml of liquid medium (with
E.  coli and with or without young worms extract) during  12
hours,  then  they  were  discarded  and newborn larvae were
transferred in next wells every day (one worm in one  well).
The  medium  with  extract  from  young  worms  was  used in
experimental  group  beginning  from  12th  day,   that   is
practically  in  postreproductive  period  of  nematode life
span.  A number of progeny was calculated  every  day.  This
investigation  was  carried  out in temperature +21=A6C and in
the darkness.  The obtained results  are  presented  in  the
following table.
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
                 Control group           Experimental group
                    n =3D 30       P             n =3D 31
                 Mean+/-S.D.                Mean+/-S.D.
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
Longevity:
mean             22,0+/-1,4    >0,05        25,5+/-1,4
maximal              39                         39
minimal              13                         14

Postreproductive
period (days)     9,5+/-1,2    >0,05        12,9+/-1,3

=46ecundity after
12th  day        0,63+/-0,22   >0,05        1,77+/-0,61
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4
Conclusion:  If the extract from young worms was applied  to
C.  elegans  during  postreproductive  period  of their life
span,  then it was able  slightly  to  increase  their  mean
longevity  (by  15,9 per cent,  not significant),  length of
postreproductive period (by 35,7 per cent, not significant),
as well as fecundity during this period.
Probably  extract  prepared from  larvae C. elegans contains
any factor, which can slightly stimulate fecundity and longe-
vity of  adult  worms,  but   this   effect   is   obviously
insufficient for noticeable prolonging of longevity.
Acknowledgment: The author wishes to express his  thanks  to
CGC for providing  C. elegans (Bristol, N2) and E. coli OP50.





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