GFP antibody staining

Garth Patterson patterson at waksman.Rutgers.EDU
Wed Aug 19 14:16:00 EST 1998


Sascha,

In Gary Ruvkun's lab, I used the clontech rabbit anti-GFP polyclonal
antibody quite a bit, and it never gave me the problem you describe.  I
found that the GFP I saw in fixed worms with the antibody matched the GFP I
saw directly in live worms.  This is using the mutated GFP from the Andy
Fire kit--the one with a change in residue 65.  I have no experience with
the souped-up clontech GFP you describe.

Garth

Garth I. Patterson
Department of Molecular Biology
Rutgers University

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