Fusion proteins in C.elegans

George Joshua george.joshua at lshtm.ac.uk
Fri Jan 23 14:19:12 EST 1998


  I'd like to confirm site of expression of a transfected gene
immunocytochemically using an antibody against the recombinant
protein.However,'am having difficulty  getting enough protein
cleaved from glutathione-s-transferase tag.Question,therefore:
  How does glutathione-s-transferase light up in immunochemical
tests in C.elegans? (ie with a view to using the fusion protein
to generate antibody).

From:   G.W.P.Joshua
        London School of Hygiene and Tropical Medicine
        Dept. Infectious and Tropical Diseases
        Keppel St.,
        London WC1E 7HT.

        Tel:  (+44)(0)171 927 2028
        FAX:  (+44)(0)171 626 8739
        E-mail:  g.joshua at lshtm.ac.uk





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